Bonin , Consuelo (2009) Analisi trascrittomatica di geni differenzialmente regolati da diverse disponibilit√† di cromato e solfato in Brassica juncea. [Laurea specialistica biennale]
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Environmental pollution by heavy metals is a global problem. Phytoremediation, the use of plants for soil cleanup, offers a cost-effective and environment-friendly alternative to conventional remediation methods. Soil and water pollution by chromium is due to the unregulated Cr-containing effluents discharged by many industrial activites. Compounds of Cr(VI), chromates and dichromates, are considered extremely toxic and harmful for linng organismus due to the transport into the cells. In this research, a cDNA-AFLP technique was used to iidentify and anlyze the expression pattern of the sulfate transporters reported in the four clusters, and of other differntially regulated genes in Brassica juncea plants grown under S availability/deprivation in presence of Cr(VI). The transcriptomic study was preceded by expression analysis of the sulfate transporter BjST1, already known in literature and by the study of physiological and biochemical parameters, like growth, sulfate uptake, chromium, sulfur, sulfate and thiols contents in plant tissue.
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