Della Regina, Orietta (2009) Effetto della competizione di molibdato e selenato sul trasporto del solfato in B. juncea. [Laurea specialistica biennale]
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Plants are able to cope with a high number of envronmental stress. in the case of heavy metals, such as chromium, molybdenum or selenium, sulphur(S) plays a fundamental role because, trough its transport systems, they can be absorbed in anion form analogous to sulphate. for the plants the sulphur represents an essential macroelements since he is assimilated and incorporated to important compound for the growth and the resistance to the stress( Schiavon and Malagoli, 2008). It is employed by the plants principally in inorganic form as sulphate ion afterwards is organicate in the molecule of the cisteyn (Cys). This amino acid is the forerunner of the metionina(Met) and other important biological compound (Brimraw, 2008). It acts, for instance, as substraum for the synthesis of the glutatione (GSH) that with metallothionine(MTs) and phitochelatine (PCs) is involved in the reply of the plant to the stress induced by heavy metals. The sulphate ion transport inner the cell and in the plant is considered the more more orderly passage of the assmilative way of sulphur. The transporters of the sulphate are coded by multigenic family that includes 14 members in A.thaliana (Hawkesford, 2003; Davidian et al.,2007), their co-ordinate expression makes an optimum management of the sulphate ion easier in the plant in different conditions of availability and supply. The study of the interactions between sulphate and oxi-anions can be useful to understand the tolerance and the accumulation capacity in the plants with the aim to improve the plant use in phytoremediation of contaminated soils. In this study was anlysed the competition betwen selenate or molybdate with the sulphate anion in plants of b. juncea. In order to evaluate effects to the exposure at the metal/loid in the short period (10 minutes-1 hour) and in the longer period (6-24 hours). Some physiological and biochemical parameters were also analysed. The absorption of the sulphate using radioactive isootope was also measured. And an analysis of genic expression of the transporter at low affinity of the sulphate BjST1 was performed by semiquantitative technical RT-PCR.
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