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Trevisan , Emanuele (2010) L'uso della genotipizzazione molecolare in ambito vegetale. [Laurea triennale]

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Abstract

The Polymerase Chain Reaction (PCR) is a molecular biology technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a routinely and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (fingerprinting and genotyping), and the detection and diagnosis of infectious diseases. Its use in so many applications is mainly due to its flexibility, velocity and reproducibility. We developed and used three different PCR-based approaches to genotype, identify and characterize three different typology of maize mutation in spontaneous, insertional and transgenic mutants.

Tipologia del documento:Laurea triennale
Corsi di Laurea Triennale:Facoltà di Agraria > Biotecnologie agrarie
Parole chiave:Genotyping, OGM, PCR
Settori scientifico-disciplinari del MIUR:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Codice ID:25429
Relatore:Varotto, Serena
Data della tesi:Settembre 2010
Biblioteca:Polo di Agripolis - Legnaro > C.I.S. di Agripolis - Biblioteca "Pietro Arduino"
Collocazione:BIO-108
Tipo di fruizione per il documento:on-line per i full-text

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