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Calore, Andrea Roberto (2012) Development of model tumor in vitro using bacterial nanocellulose. [Magistrali biennali]

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Tesi non accessibile fino a 17 July 2032 per motivi correlati alla proprietà intellettuale. Visibile a: Repository staff only

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Abstract

The aim of this work was to study the behavior of FaDu cells in contact with 2D substrates (pellicles) of BNC and to develop a 3D method to create a model of hypopharyngeal squamous carcinoma by means of BNC/alginate porous sponges. In this perspective, cells were seeded on uncoated scaffolds either as cell suspension or embedded in a 1:1 mixture of medium and Matrigel, and on scaffold pre-coated with collagen I. Nanocellulose was used also in the form of hollow tubes, filled with cell suspension and Matrigel and cultured in a stirrer flask. To create a model better resembling reality, the FaDu cells were also co-cultured with Human Umbilical Vein Endothelial Cells (HUVECs), with the expectation to observe angigogenic effects on these. The sponges seeded with Matrigel showed a higher number of cells grown inside them compared to the other seeding techniques and the gel gave good results also with the cellulose tubes. On the other hand, the co-culture gave results not as great as expected but the experiment can be improved by culturing the two cell type together for a longer time. This study shows that BNC can be used to grow in vitro 3D tumor to be used in screening of cancer therapies

Item Type:Magistrali biennali
Additional Information:Embargo per motivi di segretezza e di proprietà dei risultati e informazioni sensibili
Uncontrolled Keywords:nanocellulose, FaDu, cancer, scaffold, coculture, HUVEC
Subjects:Area 09 - Ingegneria industriale e dell'informazione > ING-INF/06 Bioingegneria elettronica e informatica
Codice ID:40186
Relatore:Ruggeri, Alfredo
Data della tesi:17 July 2012
Biblioteca:Polo di Ingegneria > Biblioteca di Ingegneria dell'Informazione e Ingegneria Elettrica "Giovanni Someda"
Tipo di fruizione per il documento:on-line per i full-text

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