Porcellato, Elena (2016) Guidance Signals in Neuronal Differantiation: biomimetic Peptides for Regenerative Medicine. [Laurea specialistica a ciclo unico]
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This thesis focuses on development and characterizaton of novel biomimetic peptides for neuronal differentiation. Based on database search, sequences homology and structural closenes, ten novel peptides - from CAM and ECM protein families: CHL1, Nerutofascin, NrCam, DCC, Robo2 and 3, LINGO2, CONTACTIN 1, 2 AND 5 were designed. Thes peptides were synthetized in SPPS, purified through RP-HPLC and their identities were confirmed by Mass Spectroscopy. Subsequent conformational analysis via CD, showed them all to assum a random coil conformation. The, thei effects on cell is tested, in order to prove their biomimetic activity. Alla novel peptides were tested on SH-SY5Y celle line to evaluate thei effect on neuronal differentiation and especally in neurite outgrowth and elongation. The entire peptide set was tested (including a number of combination) and it resulted effective.
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