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Faggiani, Alessandro (2016) Development of a model based on reaction-diffusion equations for the estimation of intracellular Ca2+ concentration. [Magistrali biennali]

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Abstract

Ca2+ ions are vital for several functions in the human body such as muscle contraction, neuronal communication, fertilization and gene transcription. The estimation of Ca2+ concentration in living cell is thus a key point in biophysical research. This thesis work is conceived to derive and validate a novel mathematical formula that, based on experimental data obtained by fluorescent Ca2+ dyes, overcomes the general approximation that Ca2+ is in equilibrium with its reactants, even in the presence of a rapid Ca2+ influx, e.g. during neuronal depolarization. Our formula, which permits to derive the intracellular Ca2+ concentration from dye fluorescence recordings and a few experimental parameters, was validated by a numerical simulation in the Matlab framework based on reaction-diffusion differential equations. At present, we are performing Ca2+ imaging experiments on HeLa cells by the UV-flash photolysis technique to obtain an experimental validation of our model. The biological target of our study would be to use the new formula to obtain a more accurate description of Ca2+ nanodomains that control neuronal activity.

Item Type:Magistrali biennali
Uncontrolled Keywords:Calcium, biophysics, imaging, flash-photolysis, simulations, validation
Subjects:Area 02 - Scienze fisiche > FIS/07 Fisica applicata (a beni culturali, ambientali, biologia e medicina)
Codice ID:53512
Relatore:Bortolozzi, Mario
Data della tesi:September 2016
Biblioteca:Polo di Scienze > Dip. Fisica e Astronomia "Galileo Galilei" - Biblioteca
Tipo di fruizione per il documento:on-line per i full-text
Tesi sperimentale (Si) o compilativa (No)?:Yes

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